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Questionnaire on T7 Bacteriophage RNA Polymerase

1. What is the molecular weight of the single-subunit RNA polymerase encoded by the T7 bacteriophage?

  • a) 50 kDa
  • b) 75 kDa
  • c) 99 kDa
  • d) 120 kDa

2. During which growth phase does the T7 RNA polymerase transcribe the phage's genes in Escherichia coli?

  • a) Lag phase
  • b) Exponential phase
  • c) Lytic growth
  • d) Stationary phase

3. Which structural superfamily does the T7 RNA polymerase belong to?

  • a) Helicases
  • b) Ligases
  • c) Polymerase superfamily
  • d) Transferases

4. Name the types of enzymes that share structural similarities with T7 RNA polymerase.

  • a) DNA polymerases
  • b) Reverse transcriptases
  • c) RNA-directed RNA polymerases
  • d) All of the above

5. How does the structure of T7 RNA polymerase compare to cellular RNA polymerases?

  • a) It is more complex and multi-subunit
  • b) It is structurally similar and multi-subunit
  • c) It is structurally unrelated and simpler
  • d) It has a higher molecular weight

6. What is the molecular weight range of more complex, multi-subunit cellular RNA polymerases?

  • a) 100-200 kDa
  • b) 200-300 kDa
  • c) 300-400 kDa
  • d) >500 kDa

7. What aspect of T7 RNA polymerase's structure has facilitated the description of its mechanism?

  • a) Its molecular weight
  • b) Its structural complexity
  • c) Its relative structural simplicity
  • d) Its multi-subunit nature

8. What is revealed by the progression through T7 RNA polymerase's transcription reaction?

  • a) Minimal conformational changes
  • b) A series of small conformational changes
  • c) A concerted series of large conformational changes
  • d) No conformational changes

9. What characteristics of T7 RNA polymerase are exploited in technologies for expressing heterologous genes?

  • a) High activity and low promoter specificity
  • b) High activity and stringent promoter specificity
  • c) Low activity and stringent promoter specificity
  • d) Low activity and low promoter specificity

10. In addition to expressing heterologous genes in vivo, for what other application is T7 RNA polymerase used?

  • a) DNA synthesis in vitro
  • b) Protein synthesis in vitro
  • c) RNA synthesis in vitro
  • d) Lipid synthesis in vitro

Answer Key:

  1. c) 99 kDa
  2. c) Lytic growth
  3. c) Polymerase superfamily
  4. d) All of the above
  5. c) It is structurally unrelated and simpler
  6. d) >500 kDa
  7. c) Its relative structural simplicity
  8. c) A concerted series of large conformational changes
  9. b) High activity and stringent promoter specificity
  10. c) RNA synthesis in vitro

https://affitechbio.com/rna-elisa-kit/affirec-recombinant-t7-polymerase-50u-l-afg-crb-3147


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